Distribution of bound and free phenolic acids in oranges (Citrus sinensis) and grapefruits (Citrus paradisi)
1991
Peleg, H. | Naim, M. | Rouseff, R.L. | Zehavi, U.
Free phenolic acids may be the precursors for vinyl phenols and off-flavours formed in citrus products during storage. Quantitative determination of free and bound phenolic acids in fruit parts of grapefruit (Citrus paradisi Macfadyen) and oranges (Citrus sinensis (L) Osbeck) was performed by extraction with ethyl acetate, silica gel column chromatography and HPLC analyses of samples before and after alkaline hydrolysis. The content of free and bound phenolic acids was further determined in juice derived from fruit harvested early, mid and late in season. As found previously for ferulic acid, phenolic acids occur mainly in bound forms in grapefruits and oranges. In both fruits the peels contained the major portion of cinnamic acids compared with the endocarp, and the flavedo was richer in hydroxycinnamic acids than albedo. In most cases, hydroxycinnamic acid content was in the following order: ferulic acid > sinapic acid > coumaric acid > caffeic acid. Results showed that the content of bound cinnamic acids was unchanged or slightly elevated from early to late season. However, the content of free acids was reduced during that period.
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