NIR transmittance estimation of free lipid content and its glycolipid and digalactosyldiglyceride contents using wheat flour lipid extracts
2000
Ohm, J.B. | Chung, O.K.
Free lipids (FL) in 72 hard winter wheat flours were extracted and dissolved in hexane. Absorbance (log 1/T) values were measured using a scanning spectrophotometer with a 2-mm cuvette, and used to develop calibration models for estimating flour FL content and glycolipid (GL) and digalactosyldiglyceride (DGDG) contents. Fifty-one calibration samples were selected based on the cutoff point of 0.3 of Mahalanobis distance, and the remaining twenty-one samples were used for validation. The best model for the estimation of FL content showed coefficients of determination (R2) of 0.95 for the calibration set and R2 = 0.89 for the validation set. Glycolipid contents could be estimated by a model which had R2 = 0.87 for the calibration set and R2 = 0.89 for the validation set. For DGDG, the best model showed R2 = 0.94 for the calibration set and R2 = 0.88 for the validation set.
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