Complexation behaviors of hyperbranched cationic glycogen derivatives with plasmid DNA revealed by resonance light scattering and circular dichroism spectroscopy
2015
Liang, Xuan | Wu, Shuyun | Ren, Xianyue | Quan, Daping | Zhang, Li‐Ming | Deng, Yubing | Yang, Liqun
A series of hyperbranched glycogen derivatives conjugated with different contents of cationic 3‐(dimethylamino)‐1‐propylamine residues, named DMAPA‐Glyp derivatives, were synthesized at room temperature. Resonance light scattering (RLS) spectroscopy was used to investigate the complexation behavior and efficacy of the DMAPA‐Glyp derivatives and plasmid DNA (pDNA). Results indicated that the DMAPA‐Glyp derivatives and pDNA molecules began to aggregate and form complexes at a weight ratio of 2. Following further increases in the DMAPA‐Glyp derivatives (w/w >2), pDNA was condensed to form compact complexes. RLS spectroscopy is thus a useful tool for studying the complexation behavior and efficacy of cationic polymers with nucleic acid molecules. Circular dichroism analysis further revealed that the conformation of pDNA transformed from B‐form to C‐form and A‐form during the complexation process.
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