Ultra-high-pressure liquid chromatography–solid-phase clean-up for determining aflatoxins in Egyptian food commodities
2015
Abdel-Azeem, S.M. | Diab, M.A. | El-Shahat, M.F.
In this work, we determined the content of regulated aflatoxins (ATs) B1, B2, G1, and G2 in food commodities using solid-phase extraction (SPE) and ultra-high-pressure liquid chromatography with fluorescence detection without derivatization. We extracted ATs from the ground samples by mixing in NaCl and 80% (v/v) methanol. The sample was enriched and cleaned up by SPE technique using Bakerbond® C18 cartridges. The extract that we obtained was immediately analyzed using isocratic elution with a mobile phase consisting of acetonitrile, methanol and deionized water in a ratio of 64:18:18. Method validation was carried out by determining these ATs in a quality control material consisting of almond T02445QC and with the add-found test. The results provided satisfactory recovery within the range of 89.6–103.3%. Repeatability and intermediate precisions were assessed as RSD (%) which were found in the range of 1.1–11.3% and 1.5–12.0%, respectively. The limit of detection (S/N=3) was 0.03, 0.02, 0.04, and 0.02μgkg−1 for B1, B2, G1 and G2, respectively. Finally, the method was successfully applied to determine ATs in raw Egyptian food commodities, namely maize, popcorn, pistachio, corn, peanuts, chilli, wheat, green coffee and almond, and the corresponding RSD did not exceed 11%.
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