Simultaneous detection of biogenic amines and aminobutyric acid isomers in high-protein forages
2019
Taubert, Johannes | Adolph, Stephanie | Scherer, Rebecca | Südekum, Karl-Heinz
High performance liquid chromatography (HPLC) was applied for simultaneous determination of selected biogenic amines and non-proteinogenic amino acids in fermented forage, i.e. silage. The four most frequently occurring biogenic amines in silage and three isomers of aminobutyric acid, potentially impairing welfare and health of farm animals, were separated in a single analysis. Without time-consuming extraction and concentration procedures, these compounds were analysed as N-substituted phenylthiocarbamyl derivatives using a reversed-phase C18 column. The method is suitable for rapid determination of the extent of amino acid decomposition during fermentation in forage with high protein content.
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