Antioxidant activity of the methanolic extract of the newly generated vegetable, baemuchae (xBrassicoraphanus)
2012
Mok, So-Youn | Choi, Mi Jin | Kim, Jongkee | Cho, Eun Ju | Lee, Sanghyun
We investigated the radical scavenging activity and protective effects of the methanol extract (ME) and ferulic acid (FA) from baemuchae (xBrassicoraphanus) against oxidative stress in vitro and on a cellular system using LLC-PK₁ renal epithelial cells. In vitro, ME and FA showed dose-dependent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. LLC-PK₁ cells displayed decreased viability in response to nitric oxide-induced oxidative stress. However, ME and FA significantly increased LLC-PK₁ cell viability in a dose-dependent manner. Based on HPLC/UV analysis, the FA content was higher in the shoot of baemuchae than in the root. The results of the present study suggest that baemuchae may be a promising source of antioxidants against oxidative stress induced by free radicals.
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