Inhibition of digestive proteases by vegetable meals in three fish species, seabream (Sparus aurata), tilapia (Oreochromis niloticus) and African sole (Solea senegalensis)
1999
Moyano Lopez, F.J. | Martinez Diaz, I. | Diaz Lopez, M. | Alarcon Lopez, F.J.
The inhibitory effect of three different vegetable foodstuffs (defatted soybean meal, corn gluten meal and wheat bran) on alkaline protease activity of seabream, tilapia and sole was evaluated. Protease inhibition on crude digestive extracts was assessed using different relative concentrations of plant meals and represented by constructing inhibition curves. SDS-PAGE zymograms were utilised to obtain further details in the characterisation of sensitivity of some fish enzymes to protease inhibitors, Tilapia showed the greatest sensitivity to protease inhibitors present in the assayed meals. A high resistance of sole digestive proteases to inhibition produced by soybean meal or corn gluten meal was detected, although they were sensitive to protease inhibitor activity in wheat bran.
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