High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
2016
Geng, Shizhong | Tian, Qin | An, Shuming | Pan, Zhiming | Chen, Xiang | Jiao, Xinan
We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The high efficiency of this genetic engineering strategy permits the study of gene function by gene knockin, site-directed mutagenesis, and gene knockout to construct live attenuated vaccines.
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