Building a better mousetrap II: Using Design of Experiments with unconfounded ions to compare the growth of different microalgae
2015
Hallenbeck, Patrick C. | Grogger, Melanie | Mraz, Megan | Veverka, Donald
A large number of unconfounded media variations were used with a Scheffe Mix Model to examine in an unambiguous fashion the effects of variations in six important ions; NH4+, NO3−, Na+, K+, PO4−, and Cl−, on the growth of Chlorella vulgaris. This allows several novel observations on media components, for example, the inhibitory effects of chloride, to be made. Using a side by side comparison, it is shown that two strains of Chlorella show significant physiological and functional differences brought out by this approach. Testing selected formulations with a diverse set of algae demonstrated different effects on both growth and cellular lipid content, in some cases driving significant lipid production. This suggests that future work using a larger portion of media composition space could lead to the development of novel media supporting maximal biomass production and lipid production.
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