Assessing effect of chromium treatment on In vitro rumen fermentation attributes
2022
Deka, R. S. | Mani, V. | Shete, S. M. | Kumar, M.
The present study was designed to investigate the effect of different levels (0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5 and 3.0 ppm) of inorganic chromium (Cr) as CrCl3•6H2O on in vitro microbial fermentation using ruminal fluid. The substrate sample used for the study consisted of a mixture of compounded concentrate, maize fodder and wheat straw in the ratio of 40:30:30, respectively. Three sets of in vitro trials in triplicate were conducted along with respective blanks to estimate the various rumen fermentation attributes such as in vitro dry matter degradability (IVDMD), in vitro organic matter degradability (IVOMD), individual volatile fatty acid (IVFA), total VFA (TVFA), partitioning factor (PF), methane (CH4) and short chain fatty acid (SCFA) to obtain the comprehensive picture of rumen fermentation pattern. Treatment with Cr did not exert any effect on IVDMD and PF values. The molar proportion of propionate at 24 h of incubation was lower (P<0.05) with 2.5 ppm of Cr compared with 1.5 ppm of Cr. However, TVFA, CH4 and SCFA did not differ across the dietary treatments. It was concluded that the addition of Cr up to 3.0 ppm did not exert any adverse effect on the studied in vitro rumen fermentation attributes.
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