Isolation and identification of an antioxidative component in canola meal
1994
Wanasundara, U. | Amarowicz, R. | Shahidi, F.
Canola meal was extracted with 95% ethanol at 80 degrees C. The dried crude extract was dissolved in methanol and fractionated on a Sephadex LH-20 column. Seven major fractions were isolated according to UV absorption and contents of phenolics and sugars. Antioxidant activity of these fractions was evaluated in a beta-carotene/linoleate model system. Fraction IV showed the best antioxidative effect by exhibiting the highest preventive activity against the bleaching of beta-carotene. This fraction was further separated by thin-layer chromatography. Four major antioxidative bands were present; the band with Rf value of 0.50 was found to be most effective in preventing oxidation of beta-carotene. This band was isolated and characterized by UV, MS, IR, (1)H NMR, and (13)C[(1)H] NMR techniques. The active compound was identified as 1-O-beta-D-glucopyranosyl sinapate.
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