Fibrivirga algicola gen. nov., sp. nov., an algicidal bacterium isolated from a freshwater river
2022
Park, Sanghwa | Cho, Ja Young | Jung, Dong-Hyun | Jang, Seok Won | Eom, Jung Hye | Nam, Seung Won | Kwon, Dae Ryul | Ryu, Jaewon | Kim, Keug Tae
An aerobic, gram-stain-negative, pink-colored, non-motile and rod-shaped algicidal bacterium, designated as JA-25ᵀ was isolated from freshwater in Geumgang River, Republic of Korea. Strain JA-25ᵀ grew at 15–30 °C and pH 6–9, and did not require NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JA-25ᵀ belongs to the family ‘Spirosomaceae’ and is most closely related to Fibrella aestuarina BUZ 2ᵀ (93.6%). Strain JA-25ᵀ showed < 90% sequence similarity to other members of the family ‘Spirosomaceae’. The average nucleotide identity(ANI), in silico DNA-DNA hybridization and average amino acid identity(AAI) values based on the genomic sequences of JA-25ᵀ and F. aestuarina BUZ 2ᵀ were 74.4, 20.5, and 73.6%, respectively. Strain JA-25ᵀ showed an algicidal effect on the marine flagellate alga Heterocapsa triquetra, but no effect on fresh water cyanobacterium (Nostoc). In genome analysis, RIPP-like peptides were detected and predicted to resemble the indolmycin biosynthetic gene cluster, which possibly influence its algicidal effect. Furthermore, a bacteriorhodopsin gene with photoheterotrophic characteristics was detected. The genomic DNA G + C content was 52.5 mol%. The major cellular fatty acids were summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:1 ω5c, C16:0 (> 10%). The major respiratory quinone was menaquinone 7 and major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two phospholipids, and five unidentified lipids. Considering the phylogenetic inference, phenotypic, and chemotaxonomic data, strain JA-25ᵀ should be classified as a novel species in the novel genus Fibrivirga, with the proposed name Fibrivirga algicola sp. nov. The type strain is JA-25ᵀ (= KCCM 43334ᵀ = NBRC 114259ᵀ).
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