Effects of genotype and explant during in vitro response to cadmium stress and variation in protein and proline contents in linseed
1997
Chakravarty, B. | Srivastava, S.
The effects of genotype and explants in influencing in vitro growth in the presence and absence of a selection pressure, viz. the heavy metal cadmium were studied. The response to callus-induction and organogenesis by different explants of three Indian cultivars of linseed (Linum usitatissimum) viz. Garima, Gaurav, and Kiran was investigated. Of the explants, hypocotyl gave the best in vitro response. Among the cultivars under control conditions (0 Cd), Gaurav and Garima fared much better in callusing response compared to Kiran, but Garima and Kiran showed higher organogenetic potential. However, in the presence of Cd (100 micromolar) callusing was not appreciably affected in any of the cultivars, but organogenesis was highly inhibited in both Garima and Gaurav; Kiran appeared to be least affected by the presence of Cd. Genotype, explant, and genotype explant interactions had highly significant effects on both callusing and organogenesis, but cadmium treatment had a greater effect on organogenesis. To estimate the stress responses due to cadmium treatment, the total protein and proline content in callus lines of the three cultivars was also studied. Both parameters showed an increase due to cadmium, but the genotypic differences in the three cultivars remained unchanged.
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