Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses
2018
Liu, Yali | Ding, Yao-Zhong | Dai, Jun-Fei | Ma, Bing | He, Ji-Jun | Ma, Wei-Min | Lv, Jian-Liang | Ma, Xiao-Yuan | Ou, Yun-Wen | Wang, Jun | Liu, Yong-Sheng | Chang, Hui-Yun | Wang, Yong-Lu | Zhang, Qiang | Liu, Xiang-Tao | Zhang, Yong-Guang | Zhang, Jie
Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.
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