Production of rhoifolin and tiliroside from callus cultures of Chorisia chodatii and Chorisia speciosa
2015
Fahim, John Refaat | Hegazi, Ghada Abd El-Moneim | Abo El-Fadl, Reda El-Said | Abd Alhady, Mohamed Reda Abd Almegid | Desoukey, Samar Yehia | Ramadan, Mahmoud Ahmed | Kamel, Mohamed Salah
The current work aims to stimulate the production of rhoifolin and tiliroside as two valuable phytochemicals from Chorisia chodatii Hassl. and Chorisia speciosa A. St.-Hil. callus cultures. A comparison between three explants from the in vitro germinated seedlings of both species for callus induction and accumulation of both flavonoids was carried out. Highly efficient calluses were induced from the leaves, stems and roots of C. chodatii seedlings on Gamborg’s B5 (B5) and Murashige and Skoog (MS) media containing 2.0mg/l β-naphthalene acetic acid (NAA) and 0.5mg/l 6-benzyladenin (BA) or kinetin (Kn), while those of C. speciosa seedlings efficiently produced calluses on both media supplemented with 0.5 or 1.0mg/l NAA and 0.5mg/l BA. Besides, the highest contents of rhoifolin (1.927mg/g DW) and tiliroside (1.776mg/g DW) from C. speciosa cultures were obtained from the calluses of seedlings’ roots and stems maintained on B5 medium containing 1.0mg/l NAA and 0.5mg/l BA, respectively. On the other hand, the maximum rhoifolin content (0.555mg/g DW) from C. chodatii cultures was obtained from the calluses of seedlings’ stems grown on B5 medium supplemented with 2.0mg/l NAA and 0.5mg/l BA, whereas the highest tiliroside content (0.547mg/g DW) was provided by the root explants on B5 medium containing 2.0mg/l NAA and 0.5mg/l Kn. Both flavonoids were bioaccumulated in greater amounts than the wild and cultivated intact plants, which provides a promising tool for their future commercial production under a controlled environment, independent of climate and soil conditions.
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