Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper
2012
Li, Zi-Cheng | An, Li-Hui | Fu, Qing | Liu, Ying | Zhang, Lei | Chen, Hao | Zhao, Xing-Ru | Wang, Li-Jing | Zheng, Bing-Hui | Zhang, Lin-Bo
The construction of a normalized cDNA library is a popular tool for identifying novel biomarkers for monitoring environmental pollution. In the present study, a normalized cDNA library was constructed from the river snail Bellamya aeruginosa after exposure to Cu2+ by using the SMART technique. The titer of the cDNA library was 1.78 × 106 pfu/ml, with a recombinant efficiency of 95.8%. In addition, from 6,000 randomly selected and sequenced clones, 5,473 high-quality ESTs were identified. After processing the sequences, 3,961 unigenes representing 897 contigs and 3,064 singlets were obtained with 27.6% redundancy. Analysis of expressed sequenced tags using COG and GO annotation and KEGG pathway data showed that a large group of genes related to growth and development, signal transduction, and defense mechanisms were present in the cDNA library. Based on our findings, this normalized cDNA library will provide a valuable resource for further research on functional genes and ecotoxicology in B. aeruginosa.
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