Continuous production of lacticin 3147 and nisin using cells immobilized in calcium alginate
2000
Scannell, A.G.M. | Hill, C. | Ross, R.P. | Marx, S. | Hartmeier, W. | Arendt, E.K.
Bacteriocinogenic strains, Lactococcus lactis subsp. lactis DPC 3147 and L. lactis DPC 496, producing lacticin 3147 and nisin, respectively, were immobilized in double-layered calcium alginate beads. These beads were inoculated into MRS broth at a ratio of 1:4 and continuously fermented for 180 h. Free cells were used to compare the effect of immobilization on bacteriocin production. After equilibrium was reached, a flow rate of 580 ml h-1 was used in the immobilized cell (IC), and 240 ml h-1 in free-cell (FC) bioreactors. Outgrowth from beads was observed after 18 h. Bacteriocin production peaked at 5120 AU ml-1 in both IC and FC bioreactors. However, FC production declined after 80 h to 160 AU ml-1 at the end of the fermentation. Results of this study indicate that immobilization offers the possibility of a more stable and long-term means of producing lacticin 3147 in laboratory media than with free cells.
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