Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food
2010
Pafundo, Simona | Gullì, Mariolina | Marmiroli, Nelson
We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin. [graphic removed]
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