Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages
2022
Stasyuk, Nataliya | Demkiv, Olha | Gayda, Galina | Zakalska, Oksana | Zakalskiy, Andriy | Serkiz, Roman | Kavetskyy, Taras | Honchar, M. M. (Mykhaĭlo Mykhaĭlovych)
Nanozymes (NZs) are nanoparticles that mimic the catalytic properties of natural enzymes. The present work aimed to obtain effective peroxidase mimetics (PO-like NZs), to characterize their morphological properties, estimate the kinetic parameters of NZs and evaluate the prospects of their application in analysis of ethanol. Herein, we have proposed a convenient spectrophotometric method for ethanol assay using reusable alginate beads enriched with alcohol oxidase (AO) and nanoparticles of PtCu (nPtCu) as PO-like NZs, and 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogen. The linear range for the proposed nPtCu–AO/alginate beads/TMB-based method is from 0.01 mM to 0.15 mM with a limit of detection of 3.3 μM ethanol. The method is used for the quantitative determination of ethanol in alcoholic beverages. The obtained results proved to be in a good correlation with the enzymatic reference method. These results highlight the potential of the nPtCu with PO-like activity in bioanalytical applications. The proposed method, being sensitive, economical and suitable for routine and micro-volume formats, can be used in clinical diagnostics for the detection of ethanol.
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