Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes
2016
Phanse, Sadhna | Wan, Cuihong | Borgeson, Blake | Tu, Fan | Drew, Kevin | Clark, Greg | Xiong, Xuejian | Kagan, Olga | Kwan, Julian | Bezginov, Alexandr | Chessman, Kyle | Pal, Swati | Cromar, Graham | Papoulas, Ophelia | Ni, Zuyao | Boutz, Daniel R. | Stoilova, Snejana | Havugimana, Pierre C. | Guo, Xinghua | Malty, Ramy H. | Sarov, Mihail | Greenblatt, Jack | Babu, Mohan | Derry, W Brent | Tillier, Elisabeth R. | Wallingford, John B. | Parkinson, John | Marcotte, Edward M. | Emili, Andrew
Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (http://www.ebi.ac.uk/pride/archive/projects/PXD002319-http://www.ebi.ac.uk/pride/archive/projects/PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].
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