Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana
1996
Kop, D.A.M van der | Schuyer, M. | Scheres, B. | Zaal, B.J. van der | Hooykaas, P.J.J.
Genes homologous to the auxin-inducible Nt103 glutathione S-transferase (GST) gene of tobacco, were isolated from a genomic library of Arabidopsis thaliana. We isolated a lambda clone containing an auxin-inducible gene, At1O3-1a, and part of a constitutively expressed gene, At103-1b. The coding regions of the Arabidopsis genes were highly homologous to each other and to the coding region of the tobacco gene but distinct from the GST genes that have been isolated from Arabidopsis thus far. Overexpression of a cDNA clone in Escherichia coli revealed that the AT103-1A protein had GST activity.
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