Simultaneous Determination of Tetrahydropalmatine, Magnolol, Emodin and Chrysophanol in Chinese Herbal Preparation by RP-HPLC-PDA
2007
Shi, Yan-Bin | Shi, Yan-Ping | Yang, Yan-Biao | Feng, Guang
A reversed phase high performance liquid chromatography coupled with photo-diode array (RP-HPLC-PDA) detection method was proposed for simultaneous determination of tetrahydropalmatine, magnolol, emodin and chrysophanol in a Chinese herbal preparation (Dan'an mixture). The separation was performed on a Diamonsil[trade mark sign] C₁₈ column (250 x 4.6 mm, 5 μm) with methanol and 0.1% phosphoric acid (88:12, v/v) as the mobile phase at the flow-rate of 0.8 mL min-¹. Two detection wavelengths were utilized for the quantitative analysis (209 nm for tetrahydropalmatine and magnolol, and 220 nm for emodin and chrysophanol, respectively). A good linear regression relationship (r >= 0.9996) between peak-areas and concentrations was obtained over the range of 0.25-50 μg mL-¹ for all the analytes. The spike recoveries, measured at three concentration levels, varied from 90.13 to 102.11%. The method was successfully applied to determine the contents of the four compounds in Dan'an mixture.
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