Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco
2007
Dun, Bao-Qing | Wang, Xu-Jing | Lu, Wei | Zhao, Zhong-Lin | Hou, Song-Na | Zhang, Bao-Ming | Li, Gui-Ying | Evans, Thomas C. Jr | Xu, Ming-Qun | Lin, Min
A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.
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