Antioxidant and Anticancer Effects of Epsilon-Poly-L-lysine Produced by Two Novel Strains of Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8
2022
Ranjbar, Hamidreza Hagh | Abari, Afrouzossadat Hosseini | Ghasemi, Seyed Mahdi | Ghorbani, Najmeh
Epsilon-Poly-L-lysine (ε-PL) is a non-toxic cationic homopolypeptide with unique bioactive properties. In this study, two novel polylysine producers, Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8 were isolated from different soil samples and identified by 16S rDNA sequence analysis. To obtain pure cationic polypeptide from these bacteria, chemical precipitation with sodium tetraphenylborate was used. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, High-performance liquid chromatography, and Fourier-transform infrared spectroscopy confirmed the purified cationic compound to be polylysine. The antioxidant activity of ε-PL was determined by the 2,2-diphenyl-1-picryl-hydrazyl-hydrate method. Moreover, the anticancer and cytotoxic effects were evaluated against MCF-7, HT-29, and L929 cells by thiazolyl blue tetrazolium bromide and flow cytometry. The maximum yields of 1.8 and 0.39 g/L polylysine were obtained by P. polymyxa HS6 and S. maltophilia YS8, respectively. The results showed that radical scavenging activity of polylysine at a minimum concentration (0.18 mg/mL) was at least 7% which increased to 90% at 8 mg/mL. Furthermore, the highest anticancer activity was observed against the MCF-7 cells (99.5%) at 0.5 mg/mL concentration, while almost no toxicity was observed towards L929 cells. Based on the results obtained in this study, it is suggested that the polylysine produced by P. polymyxa HS6 and S. maltophilia YS8 is a potential bioactive compound with significant anticancer and antioxidant properties.
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