In-vivo efficacy of poultry and fish probiotics on green mussel, perna viridis, resistance against Vibrio alginolyticus
2020
Laith, A.A. | Badr, S. | Ros-Amira, M.K. | Hassan, I.S. | Effendy, A.W.M. | Najiah, M.
The efficiency of using lactic acid bacteria derived from chicken to be used in fish to limit microbial infections in aquaculture was investigated. Gram-positive isolates were cultured on Man Rogosa Sharp agar (MRS). Identification of bacterial strains was done by analyzing nucleotide sequence of the 16S rRNA gene. Lactobacillus plantarum (98%) was isolated from Oreochromis niloticus and Enterococcus durans (96%) was isolated from Gallus gallus. The sequences were deposited in Gen Bank with the Accession No. of MF197402 and MF197403, respectively. Experimental mussels were divided into 3 groups; control group fed commercial feed only, Treatment 1 and Treatment 2 were fed with commercial feed supplemented with the candidate probiotics isolated from chicken and fish, respectively, for 21 days. P. viridis were immersed in V. alginolyticus 1.0×10⁶ CFU/ml. L. plantarum significantly affected haemocyte count, lysozyme activity and mortality rate, whereas E. durans significantly affect lysozyme activity only.
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