The development of methods for isolation and culture of preantral follicles from bovine and porcine ovaries
1996
Telfer, E.E.
The recent advances in reproductive technologies such as, in vitro fertilisation, embryo transfer, transgenesis, sexing and cloning cannot be widely applied to domestic species because of the shortage of suitable oocytes to manipulate in vitro. Current methods of superovulation and in vitro maturation of oocytes from antral follicles has not provided sufficient material to allow rapid progress. The ovaries of fetal and prepubertal animals contain a large number of preantral follicles which could be a potential supply of mature oocytes for in vitro manipulation. Culture systems that support the growth and differentiation of rodent preantral follicles have been successful at producing large numbers of meiotically competent oocytes. Methods of isolating and culturing preantral follicles from bovine and porcine ovaries are now being developed. This paper highlights the latest developments in these techniques and discusses how close we are to achieving 'oocyte fuming' for domestic species.
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