Innovative method for determining glycogen content in the Pacific oyster (Crassostrea gigas) by ion chromatography
2008
Gao, Rui-chang | Yuan, Li | Wang, Qi | Xue, Yong | Feng, Hui | Yin, Li-ang | Wang, Jing-feng | Xue, Chang-hu
A precise analytical assay was developed and validated for the determination of Pacific oyster glycogen using ion chromatography. The Pacific oyster glycogen was quantified by the determination of glucose-6-phosphate (Glc-6-P), which was derived from glucose that was hydrolyzed from glycogen. Glc-6-P, adenosine triphosphate, and adenosine diphosphate were separated by ion chromatography. The method was validated over the curve range 0.5-100mg/L for the abovementioned analytes. The recoveries were between 95% and 102%. The relative standard deviations (RSDs, c =10mg/L, n =9) were less than 4.37%. Unlike a traditional method, this validated method was inexpensive and stable.
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