Identification of dominant fungal contamination of walnut in Northwestern China and effects of storage conditions on walnut kernels
2020
Wei, L. | Fu, H. | Lin, M. | Dang, H. | Zhao, Y. | Xu, Y. | Zhang, B.
Fungal contamination of stored moldy walnuts randomly collected from different localities in northwestern China was investigated. A total of 60 samples of moldy walnuts were subjected to traditional mycological analysis for isolation of contamination fungi. The dominant species were Fusarium (20 spp.), Alternaria (10 spp.), and Penicillium (3 spp.), each with 100% frequency, followed by Aspergillus with 91.7% frequency. In total, 109 fungal species belonging to seven genera were recovered. Eighteen representative morphospecies were further identified to a named species using Internal Transcribed Spacer Identification (ITS) sequential analysis. The effect of different storage conditions (temperature, relative humidity, and moisture content) on the mildew rate of walnuts was evaluated. Whole walnut quality was maintained for 30 days at 15 °C and a moisture content of 8.53%. The effect of parameters investigated followed the sequence: moisture content > temperature > relative humidity.
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