Determination of Pirfenidone in Rat Plasma by LC-MS-MS and Its Application to a Pharmacokinetic Study
2010
Tong, Shuhua | Wang, Xianqin | Jiang, Haiyan | Xu, Xuegu | Pan, Yibin | Chen, Kunming | Wu, Jiao | Xu, Qiaoqiao | Deng, Jie | Hu, Guoxin
A simple liquid chromatography-electrospray ionization-mass spectrometric (LC-ESI-MS-MS) method was developed for determination of pirfenidone in rat plasma. After addition of phenacetin as internal standard, protein precipitation with 10% perchloric acid was employed for the sample preparation. Chromatographic separation was achieved on a C18 column, with 40: 60 (v/v) acetonitrile-0.1% formic acid as mobile phase. The assay is linear over the range 2-2,000 ng mL⁻¹, with a lower limit of quantitation of 2 ng mL⁻¹. The developed method was successfully applied to pharmacokinetic studies of pirfenidone in rats following single oral administration dose of 15 mg kg⁻¹.
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