Identification, cloning and heterologous expression of a new lectin like protein in tea
2014
Bandyopadhyay, Tirthankar | Gohain, Bornali | Bharalee, Raju | Bhagawati, Prasenjit | Kalita, Mohan Chandra | Das, Sudripta
Tea cultivation in India is greatly challenged by herbivores which render tea leaves unsuitable for any downstream processing in tea industry. Helopeltis theivora (Tea mosquito bug) is a sap sucking pest of tea leaves causing widespread tissue damage and crop loss in North eastern India. In this study we report the expression of a new variant of Ribosome inactivating protein from tea highly induced by tea mosquito bug. The heterologously expressed protein is 110 amino acids long and characterized by the presence of Q-X-W motifs and putative sugar binding sites with a molecular weight of 12.65kDa. Moreover, the study demonstrates that the expression of the encoding transcript is differentially up regulated in Helopeltis tolerant tea cultivars than the susceptible ones. The present study, for the first time reports Helopeltis induced differential up regulation of a lectin like protein in tea which may have important and positive fallout on crop improvement programmes through breeding and transgenic approaches.
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