Novel lipase from basidiomycetes Schizophyllum commune ISTL04, produced by solid state fermentation of Leucaena leucocephala seeds
2014
Singh, Manoj Kumar | Singh, Jyoti | Kumar, Madan | Thakur, Indu Shekhar
The present investigation reports a novel thermoalkalotolerant lipase producing Basidiomycetes, Schizophyllum commune ISTL04 for the first time. Abundantly available Leucaena leucocephala seeds were exploited as a cheap fermentable source for the feasible production of extracellular lipase. The isolate ISTL04 grew vigorously on Leucaena seeds in comparison to Soybean meal and Wheat bran exhibiting a lipase activity of 146.5U/g. The S. commune ISTL04 (SCI) lipase was purified to 35.76 folds with a specific activity of 238.13U/mg and an estimated mass of 60kDa. The temperature and pH optima for lipolytic activity were 60°C and 11, respectively. The enzyme exhibited remarkable stability in the presence of non-polar and polar organic solvents (50%, v/v), retaining more than 90% and 40% activity, respectively. The lipase activity increased significantly in the presence of Mg2+ (154%), Ca2+ (109.7%), Mn2+ (106%) and Tween 80 (240%). The distinctive and broader operational properties of the SCI lipase make it a promising biocatalyst for various industrial processes.
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