An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

Manderscheid, Niko; Bilyk, Bohdan; Busche, Tobias; Kalinowski, Jörn; Paululat, Thomas; Bechthold, Andreas; Petzke, Lutz; Luzhetskyy, Andriy

An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

2016

Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional φC31-attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated.

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AGROVOC Keywords

antibiotics genetically modified organisms mutagenesis secondary metabolites transposons

Bibliographic information

Published in

Journal of biotechnology

Volume 232 Pagination 110 - 117 ISSN 0168-1656

Publisher

John Wiley & Sons, Ltd

Other Subjects

Strain development; Heterologous expression; Streptomyces albus; Streptomyces albus j1074; Φc31-encoded recombination system; Multiple attachment sites; Heterologous gene expression; Antibiotic production; Multigene family

Language

English

Type

Text; Journal Article

In AGRIS since: 2024-02-28

Format: MODS

Data Provider

This bibliographic record has been provided by National Agricultural Library

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Links

DOI http://dx.doi.org/10.1016/j.jbiotec.2016.05.038

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