Utility of in vitro propagation for field-grown broccoli: effect of genotype and growing season
1993
Farnham, M.W. | Nelson, B.V.
We examined an in vitro culture method for propagating unconditioned, field-grown broccoli (Brassica oleracea L. Botrytis group) from peduncle explants by testing 20 cultivars in fall and spring. Propagation was affected significantly by genotype (cultivar) and season. The percentage of explants regenerating shoots was significantly higher for cultivars grown in spring (17% to 100%) than in fall (0% to 66%). Shoot regeneration from explants of plants within a cultivar also varied significantly (0% to 100%). Additionally, the number of propagules produced per explant was influenced by cultivar and was highly correlated with the percentage of explants regenerating shoots. This method for propagating field-grown broccoli lines is useful, but its applicability can be limited by genetic and environmental factors.
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