Analysis of physiobiochemical attributes, some key antioxidants and esculin content through HPLC in in vitro grown Cichorium intybus L. treated with ethylmethane sulfonate
2015
Hamid, Rehana | Kamili, Azra N. | Mahmooduzzafar, | Gücel, Salih | Öztürk, Münir A. (Münir Ahmet) | Ahmad, Parvaiz
This work covers studies on the effects of different concentrations of EMS (ES0, ES1, ES2, ES3, ES4, and ES5) on biochemical attributes, antioxidant enzymes and esculin content in in vitro grown Chicorium intybus. The bioactive compound esculin in in vitro raised shoots of C. intybus was determined by using high performance liquid chromatography-HPLC. EMS was used to induce in vitro mutagenesis for increase in the production of esculin content. The proline and lipid peroxidation increases with an increase in the concentration of EMS. The soluble protein, sugar content, antioxidant enzymes: superoxide dismutase, ascorbate peroxidase and glutathione reductase increase at lower concentrations of EMS and decrease at higher concentrations. EMS mutagenesis was found to be effective method for inducing increase in secondary metabolite production in C. intybus.
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