N‐hydroxysuccinimidyl p‐methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food
2014
Mazzotti, Fabio | Di Donna, Leonardo | Napoli, Anna | Aiello, Donatella | Siciliano, Carlo | Athanassopoulos, Constantinos M. | Sindona, Giovanni
We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope‐coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N‐hydroxysuccinimidyl ester of d₀/d₄‐4‐methoxybenzoic acid (d₀/d₄‐4‐MBA‐OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix‐assisted laser desorption/ionization–mass spectrometry (MS) and MS/MS. Multiple‐reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters.
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