Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa
2013
Shephard, Gordon S. | Burger, Hester-Mari | Gambacorta, Lucia | Gong, Yun Yun | Krska, R. (Rudolf) | Rheeder, John P. | Solfrizzo, Michele | Srey, Chou | Sulyok, Michael | Visconti, A. | Warth, Benedikt | van der Westhuizen, Liana
Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxin LC–MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancer region, Transkei, South Africa. Fifty-three female participants donated part of their maize-based evening meal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker) and by a ‘dilute-and-shoot’ multi-biomarker method. Results were corrected for recovery with LOD for not detected. A single biomarker method detected fumonisin B1 (FB1) (87% incidence; mean±standard deviation 0.342±0.466ng/mg creatinine) and deoxynivalenol (100%; mean 20.4±49.4ng/mg creatinine) after hydrolysis with β-glucuronidase. The multi-biomarker ‘dilute-and-shoot’ method indicated deoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with β-glucuronidase and immunoaffinity clean-up determined zearalenone (100%; 0.529±1.60ng/mg creatinine), FB1 (96%; 1.52±2.17ng/mg creatinine), α-zearalenol (92%; 0.614±1.91ng/mg creatinine), deoxynivalenol (87%; 11.3±27.1ng/mg creatinine), β-zearalenol (75%; 0.702±2.95ng/mg creatinine) and ochratoxin A (98%; 0.041±0.086ng/mg creatinine). These demonstrate the value of multi-biomarker methods in measuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinary deoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library
