Biosynthesis of silver nanoparticles by leaf extract of Albizia saman (Jacq.) Merr. and their cytotoxic effect on mitotic chromosomes of Drimia indica (Roxb.) Jessop
2017
Daphedar, Azharuddin | Taranath, Tarikeri C.
Silver nanoparticles synthesized using the leaf extract of Albizia saman (Jacq.) Merr. were tested for induction of cytogenetic abnormality in root tip cells of Drimia indica (Roxb.) Jessop (family Asperagaceae). The leaves are known to be rich in various phytochemicals like flavonoids, glycosides, saponins, steroids, tannins, and terpenoids, which may be responsible for bioreduction, biocapping, and stabilization of nanoparticles. The various instruments used for characterization include UV-VIS spectrophotometer, fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), X-Ray diffractometer (XRD), and high resolution transmission electron microscope (HR-TEM). The present study aims to evaluate the cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes by using root tip cells of D. indica. The root tips of D. indica was treated with suspensions of silver nanoparticles mixed in distilled water at different concentrations viz., 25, 50, 75, and 100% (w/v) for 6, 12, 18, and 24 h and then fixed in 1:3 ethanol: acetic acid following pre-treatment with 0.05% colchicine for cytological analysis. Silver nanoparticles induced a dose dependent decrease of mitotic index in root meristems. Furthermore, the treated meristem cells showed various types of chromosomal and mitotic aberrations such as anaphase bridge, sticky metaphase, lagging, or forward chromosome indicating genotoxic damage.
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