Variation of Soluble Proteins in Alfalfa, Sainfoin, and Birdsfoot Trefoil
1974
Gutek, L. H. | Goplen, B. P. | Howarth, R. E. | McArthur, J. M.
Soluble protein concentration was measured at different stages of growth in alfalfa (Medicago sativa L.), which does produce ruminant bloat, and in sainfoin (Onobrychis viciaefolia L.) and birdsfoot trefoil (Lotus eornieulatus L.), which do not produce ruminant bloat. Optimal sampling procedures for more effective selection of a low soluble protein (nonbloating) alfalfa in a plant breeding program was studied. Alfalfa showed no significant diurnal variation in soluble protein content. Highest levels were found in the top (10 cm) leaves and stems. In general, highest soluble protein concentrations occurred in prebud and up to the middle to late bud stage. There was significant variation due to seasonal effects. In sainfoin all of the fraction I protein was precipitated by tannins at all stages of growth. In birdsfoot trefoil accumulation of tannins resulted in decreasing soluble protein levels with advancing maturity. The development of a nonbloating, high tannin alfalfa variety was discussed.
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