Cloning of the Aspergillus niger pmrA gene, a homologue of yeast PMR1, and characterization of a pmrA null mutant
2001
Yang, J. | Kang, H.A. | Ko, S.M. | Chae, S.K. | Ryu, D.D.Y. | Kim, J.Y.
The pmr A gene, a yeast PMR1 homologue, was isolated from Aspergillus niger. Sequence analysis of the pmr A cDNA and the genomic DNA revealed that two introns exist in the coding region, and that an open reading frame in the cDNA encodes a polypeptide of 1056 amino acids containing all the conserved regions present in P-type CA2+ -ATPases. The predicted pmrA protein exhibited a high degree of sequence similarity to the Pmr1 proteins from yeasts and mammalians (50-59% identity). The expression of the pmr A cDNA partially restored the growth defect of Yarrowia lipolytica pmr 1 null mutant on EGTA-containing medium. This indicates that the A. niger pmr A gene encodes a functional homologue of the yeast P-type Ca2+ -ATPase involved in the secretory pathway. An A. niger pmr A null mutant exhibited growth retardation on EGTA-containing medium and the growth defect was overcome by adding Ca2+ or Mn2+ into the medium. This suggests an involvement of the pmrA protein in Ca2+ and Mn2+ homeostasis in A. niger.
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