Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa
2004
DeGraaf, J.D. | Berlinsky, D.L.
The aim of this study was to improve methods for short- and long-term preservation of Alantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. The effects of extender composition, cryoprotectant concentration, freezing rate, and dilution ratio on post-thaw sperm motility were examined. The fertilization capacity of fresh and post-thaw sperm were compared and sperm viability, as determined by differential fluorescent staining (propidium iodide/SYBR 14), was correlated with motility. The highest post-thaw motility was obtained when sperm was diluted 1:3 with an extender containing glutathione, sucrose, and potassium bicarbonate (modified Mounib's extender, MME), supplemented with 10% DMSO and frozen at a rate of -5 °C min-1. The post-thaw motility of cod (66 ± 2.1%) and haddock (53 ± 2.1%) sperm did not differ between 5 and 90 days of storage but was lower than fresh sperm. No difference in fertilization rate was found between fresh and post-thaw cod sperm, but fertilization was lower using post-thaw haddock sperm when compared to fresh ones. Spermatozoa motility was highly correlated (Adjusted R2=0.94) with the proportion of cells that incorporated SYBR 14. Refrigerated cod and haddock sperm diluted 1:3 with MME remained motile for 40 and 38 days, respectively. These results demonstrate that spermatozoa of cod and haddock can be refrigerated or cryopreserved and yield acceptable sperm motility and post-thaw fertilization rates.
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