Anther Culture Effectiveness in Producing Doubled Haploids of Cereals/ Putekðòu Kultûras Efektivitâte Graudaugu Dubultoto Haploîdu Izveidoðan
2014
Grauda, Dace | Miķelsone, Andra | Ļisina, Nadežda | Žagata, Kârlis | Ornicāns, Reinis | Fokina, Oksana | Lapiņa, Lita | Rashal, Isaak
Our goal was to improve the method of obtaining double haploids (DH) by anther culture from Latvian breeding material for several agriculturally important cereals in Latvia: barley, spring and winter wheat. Hybrids from Latvian breeders were initial material. It was found that copper (2.5 mg/l) added to barley anther pre-treatment media and to both barley (C3) and wheat (liquid AMC) induction media, resulted in a higher percentage of green plants-regenerants. In general, the winter wheat hybrids exhibited lower embryogenesis than spring wheat hybrids. Obtaining a large number of DH lines from barley and wheat hybrids with unknown androgenesis response can be organized in two stages: first - selection from breeding initial material hybrids responsive in anther culture, which enables production of green plants-regenerants, and second - production of DH lines in a large scale from selected hybrids.
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