Molecular Detection of Epiphytic Acaryochloris spp. on Marine Macroalgae
2006
Ohkubo, Satoshi | Miyashita, Hideaki | Murakami, Akio | Takeyama, Haruko | Tsuchiya, Tohru | Mimuro, Mamoru
A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.
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