A spectrophotometric assay for the detection of fungal peroxygenases
2012
(Martin),
Rapid and simple spectrophotometric methods are required for the unambiguous detection of recently discovered fungal peroxygenases in vivo and in vitro. This paper describes a peroxygenase-specific assay using 5-nitro-1,3-benzodioxole as substrate. The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425nm (ε₄₂₅=9,700M⁻¹cm⁻¹), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514nm (ε₅₁₄=11,400M⁻¹cm⁻¹). The assay is suitable for detecting peroxygenase activities in complex growth media and environmental samples as well as for high-throughput screenings.
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