Solid-state fermentation enhances inulinase and invertase production by Aspergillus brasiliensis
2021
Extracellular inulinase and invertase production by Aspergillus brasiliensis was evaluated in solid-state fermentation (SSF) and submerged fermentation (SmF) with a high sucrose concentration. The parameters estimated from online CO₂ analysis revealed a lower Lag phase in SSF compared to SmF; the specific CO₂ production rate (μCO₂) decreased in both systems as the sucrose concentration increased. The highest maximum CO₂ production rate (10.45 ± 0.72 mg/h mL) and total CO₂ production (138.53 ± 6.48 mg/mL) were obtained in SSF with a sucrose concentration of 210 g/L. Inulinase and invertase production (1.13 ± 0.02 and 5.84 ± 0.12 U/mL, respectively) in SmF was independent of the initial sucrose concentration; meanwhile, both activities increased in SSF as the sucrose concentration increased, reaching maximum values of 5.88 ± 0.78 and 41.69 ± 3.60 U/mL, respectively, at the highest sucrose concentration (210 g/L). The zymographic analysis showed one band of inulinase and two bands of invertases involved in sucrose hydrolysis.
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