Acetone stimulation of ethanol production from D-xylose by Pachysolen tannophilus
1986
Alexander, N.J.
Pachysolen tannophilus, a homothallic yeast, converts xylose to ethanol at a yield of 0.3 (g/g xylose). Concomitant with ethanol production, xylitol accumulates in the culture medium at similar yields (0.3 g/g xylose). The addition of the hydrogen-accepting compound, acetone, increases the amount of ethanol produced by this organism by 50-70%. The increase in ethanol is directly correlated with a decrease in xylitol secreted. The results indicate that conversion of acetone to 2-propanol by the cells provides the NAD+ used as a cofactor by xylitol dehydrogenase, the enzyme responsible for converting xylitol to xylulose.
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