On-line Method for Montelukast Determination in Bile Salt Medium with Multivariate Optimization and Fluorescent Detection
2022
The present development involved a flow injection strategy using a mini-column of multiwalled carbon nanotubes and fluorescent detection (λₑₓ of 283 nm, λₑₘ of 397 nm) for montelukast (MKT) determination. Optimization technique through experimental design was implemented in order to consider simultaneously different aspects of analysis. Different factors that influence the evaluated analytical responses (sensitivity and sampling frequency) were studied. Taurodeoxycholate bile salt was evaluated as enhancing agent on MKT fluorescence signal. The optimal conditions for MKT determination were sample taurodeoxycholate concentration of 50 μM, eluent flow rate of 0.6 mL/min and sample flow rate of 2.2 mL/min. The method was confirmed to be linear in the range of 0.03–15 µg/mL, with the limit of detection of 11 ng/mL and the limit of quantification of 30 ng/mL. The method was successfully applied to MKT determination in pharmaceutical preparations with suitable recoveries between 93 and 108%.
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