Protoplast induction from sporophyte tissues of the heterosporous fern Azolla
1987
We have developed a method for producing protoplasts from the heterosporous water fern Azolla using a combination of Cellulysin (4.0%) and Pectolyase (0.025%) in 0.6 M mannitol containing 6 mM CaCl2 2H2O. These protoplasts regenerate new cell walls within 48 hours when cultured on modified Gamborg B-5 medium.
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Bibliographic information
Publisher
The Royal Society of Chemistry
Other Subjects
Ferns and fern allies; Plant production (horticultural crops); Dose response; Cell wall components; Plant physiology and biochemistry; In vitro digestion; Pectin lyase
Language
English
Note
Includes references
2019-12-05
Type
Journal Article; Text
2024-02-28
MODS
Data Provider
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