Lignin antioxidants extracted from lignocellulosic biomasses by treatment with ammonia water
2015
Kurakake, Masahiro | Hirotsu, Saya | Shibata, Miyuki | Kubota, Asami | Makino, Atsushi
In this study, it was found out that treatment with ammonia water efficiently produced thermostable antioxidant from lignin of selected herbaceous and soft wood species. The SOD-like activity was enhanced by ammonia water treatment and Madake (Phyllostachys bambusoides), Moso (Phyllostachys edulis) and Susuki (Miscanthus sinensis) showed high activity among the herbaceous species. The IC50s for the SOD-like activity of the lignin extract of Akamatsu (Pinus densiflora) and Moso were 0.0190 and 0.0097mg/ml, respectively, where the IC50 value was represented using the total phenolic content. The lignin antioxidants extracted from Moso and Akamatsu had high thermostablility. This pretreatment also improved enzymatic hydrolysis of cellulose and hemicellulose for herbaceous species. After enzymatic hydrolysis of the treated samples, the release of glucose from Moso was about 3.5 fold more than the untreated, achieving total sugars of 114.6mg/g-sample. Pretreatment of Akamatsu with high lignin content did not provide any benefits.
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