Initiation of carp spermatozoa motility and early ATP reduction after milt contamination by urine
1998
Poupard, G.P. | Paxion, C. | Cosson, J. | Jeulin, C. | Fierville, F. | Billard, R.
Fish sperm collected by stripping males is frequently contaminated by urine. In this study, carp milt mixed with urine (0.5-7.5% of volume) was studied in order to evaluate the changes of some motility parameters (percentage of motile spermatozoa, velocity and beat frequency) and the ATP content of spermatozoa. In the absence of urine contamination, spermatozoa had an ATP content in the range of 8-9 nmol/10(8) spermatozoa, an initial velocity of 100-160 micrometers s-1 and a flagellar beat frequency around 30-50 Hz, 10 s after a 1/2000 dilution in an activating medium (45 mM NaCl, 5 mM KCl, 30 mM Tris-HCl pH 8.0, osmolality < 160 mosM kg-1). In contrast, when milt was contaminated with 7.5% of urine for 1 h, the ATP content was 4-5 nmol/10(8) spermatozoa and most spermatozoa had low initial velocity (30-100 micrometers s-1) and flagellar beat frequency (10-30 Hz). It appears that the low osmolality of urine was responsible for the degradation in the quality of carp spermatozoa by an early activation in the collecting tube which induced an early reduction of the intracellular ATP store. From a practical point of view, milt contamination by urine during stripping can be avoided by first pressing the abdomen before sampling and then collecting the remaining urine by means of a catheter introduced into the urinary bladder.
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