Preparation and application of a beta-d-glucan microsphere conjugated protein A/G
2020
Yang, Zishan | Sun, Aiping | Zhao, Xiaoshuai | Song, Mingyang | Wei, Jingjing | Wang, Jingyuan | Zhao, Tiesuo | Xie, Yunfei | Chen, Zhiguo | Tian, Zhongwei | Liu, Hui | Huang, Zhen | Song, Xiangfeng | Feng, Zhiwei
Antibodies (Abs) have been widely used in both immunodiagnostics and immunotherapy for the treatment of various diseases and, in recent years, scientific research applications. With the increasing use of Abs, there has been an urgent demand for low-cost and highly efficient purification methods. In this study, we present a novel formulation based on a β-d-glucan particle loaded protein A/G (GP-protein A/G conjugates) by the carbodiimide method for the purification of immunoglobulin (IgG) antibodies. The prepared GP-protein A/G conjugates exhibit high stability and isolation efficiency. The microspheres also constitute an essential specialty reagent useful for isolating IgG from mammalian species such as goat, mouse and rabbit. Recovery of IgG showed that up to a purity of 92% was reached in the elution step. In addition, they has been shown to be important tools for molecular purification methods such as immunoprecipitation and co-immunoprecipitation. Taken together, these results suggest that the GP-protein A/G system has the potential to be used as a platform for purification techniques.
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